Cell culture refers to the isolation of cells from an animal or plant and their subsequent growth in a favourable artificial environment. Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, and mutagenesis and carcinogenesis. It is also used in drug screening and development, and large scale manufacturing of biological compounds (e.g., vaccines, therapeutic proteins). The major advantage of using cell culture for any of these applications is the consistency and reproducibility of results that can be obtained from using a batch of clonal cells. The cell culture house facility of ISF College of pharmacy was established in April 2010, inaugurated by Prof. (Dr) Buta Singh, Vice Chancellor, Maharaja Ranjit Singh Punjab Technical University, Bathinda. The cross contamination in the cell culture were controlled using Air flow HEPA filter cabinet at the entrance of the facility and utilization of UV lamp throughout the cell culture facility.The cell culture facility having sophisticated instruments such BCL2 level biosafety cabinet, CO2 incubators, BOD incubator, fluorescence microscope and flow cytometer which are eligible to carry out all type of in vitro assays on primary and secondary cell lines.\nCell culture facility has a separate room along with air conditioning, sterilization and facility as per follow-\n
\n\u2022\tAseptic Room for Biosafety cabinet, CO2 incubator and Fluorescence microscopy\n<\/p>\n
\u2022\tFreezer (-80C) for storage of mammalian cells\n<\/p>\n
\u2022\tQuarantine room<\/p>\n\n\n\n\n\n\n
In-vitro studies of Drug formulation, Drug candidate, Chemical entities, and Plant extract<\/p>
\n
(I)\tAnti-Cancer Studies on various cell lines:<\/strong><\/p>\n (II)\tAnti-inflammatory and Protection Studies against toxic substances and chemical of Drug formulation, Drug candidate, Chemical entities, and Plant extract (On Human and mice cells)<\/p>\n Assays that can be performed:<\/b><\/p>\n Associate Professor<\/span><\/p>\n\n In charge Cell Culture Facility<\/span><\/p>\n\n ISF College of Pharmacy<\/span>(An\nAutonomous College)<\/span><\/span><\/p>\n\n NAAC Accredited “A” Grade College<\/span><\/p>\n\n\n\n Mobile- 7986421860<\/span><\/p>\n\n Email-rajveer@isfcp.org<\/span><\/p>\n\n\n\n\n\n\n\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"pagelayer_contact_templates":[],"_pagelayer_content":"","footnotes":""},"class_list":["post-5734","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/pages\/5734","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/comments?post=5734"}],"version-history":[{"count":5,"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/pages\/5734\/revisions"}],"predecessor-version":[{"id":5742,"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/pages\/5734\/revisions\/5742"}],"wp:attachment":[{"href":"https:\/\/isfcp.org\/i\/wp-json\/wp\/v2\/media?parent=5734"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
1.\tHepatic Cancer\n
2.\tBreast Cancer\n
3.\tLung Cancer\n
4.\tColon Cancer\n
5.\tBrainCancer (Glioblastoma&Neuroblastoma)\n
6.\t Ovarian Cancer\n
7.\t Renal Cancer\n
8.\tProstate Cancer\n
9.\t Pancreatic Cancer\n\n
\n(III)\tAnti-diabetic Studies of Drug formulation, Drug candidate, Chemical entities, and Plant extract\n\n(IV)\tAnti-Malarial Studies of Drug formulation, Drug candidate, Chemical entities, and Plant extract\n\n(V)\tAnti- Parkinson\u2019s and Anti-Alzheimer\u2019s Studies of Drug formulation, Drug candidate, Chemical entities, and Plant extract\n
\u2022\tCell Viability Assay\n
\u2022\tROS assay\n
\u2022\tNO assay\n
\u2022\tInterleukin screening\n
\u2022\tMitochondria degradation assay \n
\u2022\tELISA \n
\u2022\tApoptosis Assay by FACS\n
\u2022\tCell cycle Assay by FACS\n
\u2022\tImmunofluorescence and Translocation assay by FACS and Fluorescence microscopy.\n
\u2022\tWestern Blotting\n
\u2022\tTransfection study (Lipofectamine based).\n
\u2022\tGeneration of cell based model by siRNA, and transfection.\n
\u2022\tTarget based study based upon disease type.<\/p>\n\n\n\n\n\n\n
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